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Comparison of conventional susceptibility testing, penicillin-binding protein 2a latex agglutination testing, and mecA real-time PCR for detection of oxacillin resistance in Staphylococcus aureus and coagulase-negative Staphylococcus.

Miller MB, Meyer H, Rogers E, Gilligan PH

University of North Carolina Hospitals, University of North Carolina School of Medicine, Campus Box 7525, Chapel Hill, NC 27599-7525, USA. mbmiller@unch.unc.edu

Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 microl greatly reduced the number of isolates requiring induction.

Published 7 July 2005 in J Clin Microbiol, 43(7): 3450-2.
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