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Shortcut method for extraction of Staphylococcus aureus DNA from blood cultures and conventional cultures for use in real-time PCR assays.

Adams DN

Department of Microbiology, Royal Prince Alfred Hospital, Missenden Rd., Camperdown 2050, NSW, Australia. nick.adams@email.cs.nsw.gov.au

PCR allows rapid determination of both the presence of Staphylococcus aureus and of methicillin resistance. I present here a simple method for direct extraction from blood cultures or colonies for use in real-time PCR, eliminating tedious, time-consuming methods of DNA extraction which may otherwise deter microbiology labs from offering PCR.

Published 15 June 2005 in J Clin Microbiol, 43(6): 2932-3.
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