Staphylococcus Research Today is a free monthly online journal that collates and summarizes the latest research about Staphylococcus, including details on mrsa, hospitals, infection, antibiotic resistance, superbugs.

Kinetic characterization of the glycosyltransferase module of Staphylococcus aureus PBP2.

Barrett D, Leimkuhler C, Chen L, Walker D, Kahne D, Walker S

Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA.

We report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.

Published 3 March 2005 in J Bacteriol, 187(6): 2215-7.
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