Staphylococcus Research Today is a free monthly online journal that collates and summarizes the latest research about Staphylococcus, including details on mrsa, hospitals, infection, antibiotic resistance, superbugs.

High-density DNA probe arrays for identification of staphylococci to the species level.

Couzinet S, Jay C, Barras C, Vachon R, Vernet G, Ninet B, Jan I, Minazio MA, Francois P, Lew D, Troesch A, Schrenzel J

Genomic Research Laboratory, University Hospitals of Geneva Rue Micheli-du-Crest 24,1211 Geneva 14, Switzerland.

Rapid and accurate identification and speciation of staphylococci clinical isolates is important for predicting medical pathology. We evaluated the ability of a high-density DNA probe array based on 16S rDNA sequences to identify Staphylococcus species. Correct identification was observed for 185 out of the 201 strains (92%). Of the 33 tested species, the array was able to correctly identify 30 of them. The total time required for identification of 4 isolates was 5 h. Such a tool represents a powerful method for routine microbiological diagnostic as well as for epidemiological studies.

Published 21 February 2005 in J Microbiol Methods, 61(2): 201-8.
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